National Institute on Aging
National Institutes of Health
NIA Home
Research Programs
Intramural
NIA Intramural Research Program
Laboratory of Genetics
separator
RNAi

separator
RNAi is a set of fluorescence microscopy images of fly cells (D. melanogaster) subjected to a set of gene-knockdowns using RNAi. The cells are stained with DAPI to visualize their nuclei. Each class contains 1024x1024 TIFF images of phenotypes resulting from knock-down of a particular gene. Ten genes were selected and their Gene IDs are used for the class names.
The images were acquired automatically using a DeltaVision light microscope with a 60x objective. Each image is produced by deconvolution followed by maximum intensity projection (MIP) of a stack of 11 images at different focal planes.
This dataset can be used as more than a simple classification problem because similarity between phenotypes is also of considerable importance. Different genes may be part of the same cellular mechanism, and therefore may produce phenotypes that are more similar to each other than phenotypes from other genes.
This type of analysis can be used to find similarities between genes based on the phenotypes that they produce, in contrast to finding similarities between genes based only on their sequences using algorithms such as BLAST.

The classes are:

CG1258

CG3733

CG3938

CG7922

CG8114

CG10873

CG12284

CG17161

CG8222

CG9484


The source for this dataset is Mark Eckley (NIA).

Download this dataset

Wndchrm performance report using RNAi